AP4 method for two-tube nested PCR detection of AHPND isolates of Vibrio parahaemolyticus
Our previous work on the mechanism of virulence for the unique isolates of Vibrio parahaemolyticus that cause acute hepatopancreatic necrosis disease (VPAHPND) revealed that it was mediated by a binary Pir-like toxin pair ToxA and ToxB.These toxins are located on the pVA plasmid, a plasmid carried by AHPND-causing strain of V.parahaemolyticus with